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Rene E. Harrison

B.Sc., M.Sc., Ph.D.

Contact

Office:S427C    Phone:  x7377

Lab:S422         Phone:  x7440

Email:  harrison@utsc.utoronto.ca

Research

Our lab is studying the microtubule cytoskeleton.  We are interested in microtubule dynamics and microtubule regulating proteins in the immune cell, macrophages and the bone cell, osteoclasts.  Macrophages are the key cell responsible for degradation of pathogens by a process known as phagocytosis.  Osteoclasts are equally destructive cells, charged with degrading the dense calcified bone matrix.  We are interested how the microtubule cytoskeleton contributes to the function of these cell types, and particularly how the microtubule cytoskeleton is modulated during invasion of pathogens and in bone-wasting disorders.  Recently, we are also investigating how the cytoskeleton of bone cells is affected in microgravity environments.


Laboratory Group

Arian Khandani, Lab Technician
Prerna Patel, Postdoctoral Fellow
Marcelo Binker, Postdoctoral Fellow
Edward Eng, Ph.D. candidate
Kristen Silver, Ph.D. candidate
Noushin Nabavi, Ph.D. candidate
Sophie Pang, M.Sc. candidate
William Poggemiller, D01 student
Jemy Joseph, D01 student
Kristine Louis, D01 student
Ajay Bhargava, Volunteer
Puneet Kaur, Volunteer
Kriti Arora, Volunteer

Courses

BGYB10 - Cell Biology

BGYD23 - Special Topics in Cell Biology and Molecular Genetics

Publications

Khandani A, Eng E, Jongstra-Bilen J, Schreiber AD, Douda D, Samavarchi-Tehrani P, Harrison RE. Microtubules regulate PI-3K activity and recruitment to the phagocytic cup during Fc{gamma} receptor-mediated phagocytosis in nonelicited macrophages. J Leukoc Biol. 2007 May 14

Eng EW, Bettio A, Ibrahim J, Harrison RE. MTOC Reorientation Occurs during Fc{gamma}R-mediated Phagocytosis in Macrophages. Mol Biol Cell. 2007 Apr 18

Touret N, Paroutis P, Terebiznik M, Harrison RE, Trombetta S, Pypaert M, Chow A, Jiang A, Shaw J, Yip C, Moore HP, van der Wel N, Houben D, Peters PJ, de Chastellier C, Mellman I, Grinstein S. Quantitative and dynamic assessment of the contribution of the ER to phagosome formation. Cell. 2005 Oct 7;123(1):157-70.

Harrison RE, Brumell JH, Khandani A, Bucci C, Scott CC, Jiang X, Finlay BB, Grinstein S. Salmonella impairs RILP recruitment to Rab7 during maturation of invasion vacuoles.
Mol Biol Cell. 2004 Jul;15(7):3146-54.

Vieira OV, Harrison RE, Scott CC, Stenmark H, Alexander D, Liu J, Gruenberg J, Schreiber AD, Grinstein S. Acquisition of Hrs, an essential component of phagosomal maturation, is impaired by mycobacteria. Mol Cell Biol. 2004 May;24(10):4593-604.

Harrison RE, Sikorski BA, Jongstra J. Leukocyte-specific protein 1 targets the ERK/MAP kinase scaffold protein KSR and MEK1 and ERK2 to the actin cytoskeleton.
J Cell Sci. 2004 Apr 15;117(Pt 10):2151-7.

Lee WL, Harrison RE, Grinstein S. Phagocytosis by neutrophils. Microbes Infect. 2003 Nov;5(14):1299-306. Review.

Harrison RE, Bucci C, Vieira OV, Schroer TA, Grinstein S. Phagosomes fuse with late endosomes and/or lysosomes by extension of membrane protrusions along microtubules: role of Rab7 and RILP. Mol Cell Biol. 2003 Sep;23(18):6494-506.

Vieira OV, Bucci C, Harrison RE, Trimble WS, Lanzetti L, Gruenberg J, Schreiber AD, Stahl PD, Grinstein S. Modulation of Rab5 and Rab7 recruitment to phagosomes by phosphatidylinositol 3-kinase. Mol Cell Biol. 2003 Apr;23(7):2501-14.

Harrison RE, Grinstein S. Phagocytosis and the microtubule cytoskeleton. Biochem Cell Biol. 2002;80(5):509-15. Review.

Harrison RE, Touret N, Grinstein S. Microbial killing: oxidants, proteases and ions.
Curr Biol. 2002 May 14;12(10):R357-9.

Harrison RE, Turley EA. Active erk regulates microtubule stability in H-ras-transformed cells. Neoplasia. 2001 Sep-Oct;3(5):385-94.

Harrison RE, Huebner E. Unipolar microtubule array is directly involved in nurse cell-oocyte transport. Cell Motil Cytoskeleton. 1997;36(4):355-62.


 
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