Clickable 5′-γ-Ferrocenyl Adenosine Triphosphate Bioconjugates in Kinase-Catalyzed Phosphorylations
Dr. Nan Wang, Dr. Zhe She, Yen-Chun Lin, Prof. Sanela Martić, Dr. David J. Mann and Prof. Heinz-Bernhard Kraatz
A clickable site has been introduced into a tri-functional 5′-γ-ferrocenyl (Fc) adenosine triphosphate (Fc-ATP) derivative, which has been used as an effective co-substrate for kinase-catalyzed phosphorylation. The compound was also applied to the Fc-Ab1/Ab2 system, providing both electrochemical results and immunodetection. The clickable reaction site makes direct modification possible, which greatly expands its application.